Hoʻohana maʻamau o nā pā ELISA

Ma ke ʻano he mea hana hoʻokolohua, ʻo ke ʻano kumu o kaELISA pāhe pūʻulu microplates i loaʻa nā mea paʻa paʻa (e like me nā proteins a me nā antibodies). I ka hoʻohana ʻana i ka pā ELISA, e hoʻopili ʻia ka laʻana e hoʻāʻo ʻia me kahi molela i kapa inoa ʻia e ka enzyme, a laila e hana ʻia kahi hoʻololi kala ʻike ʻia ma o ka hoʻohui ʻana i kahi substrate matrix, a e helu ʻia ka ʻike a i ʻole ka hana o ka molekele target. a i ʻole loiloi ʻia ma ka ʻike ʻana i ka hōʻailona hōʻailona a i ʻole fluorescence. Eia nā noi maʻamau o nā papa ELISA ma nā ʻano like ʻole:

1. Protein quantitative analysis: Hiki ke hoʻohana ʻia nā papa ELISA e ana i ka manaʻo a me ka hana o nā protein i loko o nā laʻana olaola e like me ka serum a me nā cell supernatants, e hāʻawi ana i nā mea hana ikaika no ka ʻike ʻana i nā hōʻailona tumo, hepatitis virus antibodies, myocardial injury markers, etc. a me ke kōkua ʻana i nā kauka i ka ʻike mua ʻana a me ka nānā ʻana i nā maʻi.

2. Ka nānā 'ana i ka cytokine: Ma ka noiʻi immunology,Nā papa ELISAHiki iā ia ke ana i nā pae cytokine i loko o nā supernatants moʻomeheu cell a i ʻole nā ​​wai kiko, e kōkua ana i ka hoʻomaopopo ʻana i nā kaʻina hana olaola e like me nā pane immune a me nā pane inflammatory, a he mea nui ia no ka hoʻomohala ʻana i nā lāʻau lapaʻau hou a me nā lāʻau lapaʻau.

3. Ka noiʻi nucleic acid: Ma o nā papa ELISA, hiki i nā ʻepekema ke ʻike a kālailai i ka ʻike a me ka hana o DNA a i ʻole RNA, hāʻawi i ke kākoʻo ʻikepili no ka noiʻi molecular biology e like me ka gene expression a me ka hoʻoponopono gene, a hoʻoikaika hou i ka hoʻomohala ʻana i nā kahua e like me ka gene therapy. a me ka hoʻoponopono gene.

4. Ka noiʻi hana enzyme: Hiki i nā papa ELISA ke ana pololei i ka hana enzyme, kōkua i ka poʻe noiʻi e hoʻomaopopo i ka hana a me ka hoʻoponopono ʻana o nā enzymes i loko o nā mea ola, a hāʻawi i nā kuhikuhi koʻikoʻi no ka noiʻi ʻana i ka ʻenekinia enzyme, metabolic engineering a me nā kula ʻē aʻe.

5. Ke noiʻi ʻana i ka pilina pili waena:Nā papa ELISAhiki ke hoʻohana ʻia ʻaʻole wale e ana i ka ʻike o nā molekala, akā e aʻo pū i ka pilina ma waena o nā mole. Ma ka hoʻohui ʻana i nā ʻenehana e like me ka surface plasmon resonance a me ka fluorescence resonance energy transfer, hiki ke nānā ʻia ke kaʻina hana hoʻopaʻa a me ka dissociation ma waena o nā molekala i ka manawa maoli, e hāʻawi ana i nā hiʻohiʻona hou a me nā ala no ka hoʻolālā lāʻau, ka pilina protein a me nā noiʻi ʻē aʻe.