Hiki Hou | KUAI | Nā Papa ʻEleʻele Elisa

Ma nā ʻano like ʻole o ka ʻepekema ola, ʻo ka hoʻoholo ʻana i ka manawa, kūpono a me ka hoʻokele waiwai a me ka helu ʻana o nā antigens a i ʻole nā ​​antibodies i loaʻa i kahi laʻana he mea koʻikoʻi.

Ua hōʻike ʻia ka Enzyme-linked immunosorbent assay (ELISA) he noiʻi waiwai nui a me ka mea hana diagnostic no ke ana ʻana i nā antibodies a i ʻole antigens i loko o nā laʻana olaola ma o ka adsorption o nā antigens i ʻike ʻia a i ʻole nā ​​antibodies ma luna o ka ʻili o kahi mea lawe paʻa paʻa, e hiki ai i ka enzyme ( ʻo ka nui o ka HRP)-labeled antigen-antibody reactions ma ka ʻili paʻa. Hiki ke hoʻohana ʻia kēia ʻenehana no ka ʻike ʻana i nā antigens mole nui a me nā antibodies kikoʻī, a me nā mea ʻē aʻe. Eia naʻe, ua kaupalena ʻia ka ʻike a me ka ikaika o ka ʻike ELISA e nā hemahema o ka ʻenehana absorption māmā ma muli o ka mana nui o nā kūlana o waho i ka hoʻololi kala o ka hopena a me ka haʻahaʻa haʻahaʻa haʻahaʻa o ka waiwai OD.

ʻO ka ʻenehana DELFIA ---- ʻo ia wale nō ke hoʻololi i ka enzyme HRP me ka lanthanide chelate (Eu, Sm, Tb, Dy) e hōʻailona ana i ka antibody ʻike ma nā hoʻokolohua ELISA kuʻuna. ʻO nā lanthanides i hoʻohana ʻia ma DELFIA kahi papa kūikawā o nā mea fluorescent, kahi e koi ai i nā mea hoʻokolohua --- nā papa Elisa. Loaʻa i nā Lanthanides nā ola fluorescence o microseconds a i ʻole milliseconds, i hui pū ʻia me ka ʻike i hoʻoholo ʻia i ka manawa e hōʻemi nui ai i ka hoʻopili ʻana i ka autofluorescence kāʻei kua, a ʻo kā lākou ākea Strokes 'hoʻonui nui i ka naʻau o ka assay.

ʻO ka hapa nui o ka ELISA e koho i ka pāpaʻi hōʻailona enzyme e like me ka mea lawe a me ka pahu, akā ʻo ke kukui i hoʻokuʻu ʻia i ka hopena luminescence he isotropic, ʻaʻole e hoʻopuehu wale ʻia ke kukui mai ka ʻaoʻao kūpaʻa, akā e hoʻopuehu pū ʻia mai ka ʻaoʻao ākea. e hele maʻalahi ma waena o nā puka like ʻole o ka pāpaʻi hōʻailona enzyme akaka a me ka paia o nā lua. Hoʻopili ʻia nā puka pili i kekahi i kekahi a pili i nā hopena hoʻokolohua.

Hiki ke hoʻohana ʻia nā Papa Elisa Keʻokeʻo no ka ʻike ʻana i nā kukui nāwaliwali a hoʻohana mau ʻia no ka chemiluminescence maʻamau a me ka hoʻomohala ʻana o ka waihoʻoluʻu substrate (e laʻa.

ʻOi aku ka nāwaliwali o ka ʻeleʻele Elisa Plates ma mua o nā papa lepili enzyme keʻokeʻo ma muli o kā lākou lawe māmā ʻana, a hoʻohana mau ʻia no ka ʻike ʻana i nā kukui ikaika, e like me ka ʻike fluorescence.

Nā Pōmaikaʻi o nā Papa Cotaus®Elisa

● Paʻa kiʻekiʻe

ʻO nā papa Cotaus®Elisa me ka ʻeleʻele ʻeleʻele i hana ʻia me nā mea non-fluorescent ponoʻī, ua mālama ʻia ka ʻili e hoʻomaikaʻi nui i kona hiki ke hoʻopaʻa i ka protein, hiki ke hiki i 500ng IgG/cm2, a ʻo ke kaumaha mole o nā protein paʻa nui he> 10kD. .

● Hoʻopau ka fluorescence haʻahaʻa haʻahaʻa i nā pilikia i hana ʻia e nā hopena kikoʻī ʻole.

Hiki i nā pahu ʻeleʻele ke hoʻopau i ka fluorescence hoʻonāwaliwali i ka hope no ka mea e loaʻa iā ia kona māmā māmā.

● Hoʻolālā hiki ke wehe

ʻOi aku ka maʻalahi o ka hoʻolālā detachable o ke keʻokeʻo enzyme plate frame a me nā papa enzyme ʻeleʻele no ka hana. E noʻonoʻo i ka hana hoʻokaʻawale, mai hoʻoikaika i ka haki ʻana i kekahi hopena, i ʻole e maʻalahi ka haki.

Hoʻokaʻawale Huahana

No nā kikoʻī huahana hou aku, e ʻoluʻolu e kelepona mai iā mākou